Molecular Fluorescence Instrumental : Part 2: Actual Excitation and Emission Maxima |
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date: 22-4-2020
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Date: 2-2-2020
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Date: 22-2-2020
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Date: 4-2-2020
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In this part of the experiment, you will determine the optimal instrument operation parameters. Using the approximate excitation wavelengths determined above from the UV-Vis absorbance spectrum, obtain two emission spectra. The emission maximum observed in the two emission spectra will then be used to collect an excitation spectrum to "refine" the choice of excitation wavelength. You will see that the fluorescence excitation spectrum closely follows the absorbance spectrum.
You should collect the following spectra:
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علامات بسيطة في جسدك قد تنذر بمرض "قاتل"
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أول صور ثلاثية الأبعاد للغدة الزعترية البشرية
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مكتبة أمّ البنين النسويّة تصدر العدد 212 من مجلّة رياض الزهراء (عليها السلام)
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