Eukaryote Genomes

Cellular genomes (i.e. non-viral) vary tremendously in size and complexity. The smallest known from a prokaryote (bacterium) contains about 600 000 DNA base pairs; in contrast, eukaryote genomes such as human and mouse genomes have some 3 billion DNA base pairs (Table ). In eukaryotes, genomic analysis is frequently complicated by their polyploid nature. In humans and the majority of the animal kingdom, including ‘model species’ such as mice and fruit flies, the DNA is packaged into two sets of chromosomes, one set being inherited from each parent. The human diploid genome is organised into 23 pairs of linear chromosomes, one set being inherited from each parent.

All pairs of metaphase chromosomes appear identical in normal karyotypes apart from the sex chromosomes, X and Y, both of which are present in male cells. Sometimes gene pairs differ in their DNA sequences to a small degree and these ‘allelic’ differences can have a marked effect on the expression of the ‘phenotype’. The effects of such allelic differences, which could be as small as the replacement of a single nucleotide within the amino acid coding portion of the gene or in the associated non-coding DNA that regulates gene action, give rise to mendelian genetic phenomena referred to as ‘dominant’, ‘recessive’ or ‘codominant’.
Detecting and characterising the molecular features that differentiate alleles and their expression have remained an important quest for genome scientists and geneticists.

Table : Approximate haploid genome sizes in various organisms

مواضيع ذات صلة

الإضعاف :التوقف المبكر للاستنساخ (Attenuation: the premature termination of transcription)

كابح التربتوفان tryptophan repressor

Gel Electrophoresis Assay of Eukaryotic Replication Origins

Constancy of the E. coli DNA Elongation Rate

Bidirectional Replication from E. coli Origins

أوبرون اللاكتوز lac operon

اكتشاف الأوبرون

مثبطات تخليق البروتين .... وسيلة للعلاج

تحوير سلاسل الأحماض الأمينية الجانبية للبروتين (Amino acids side chains modifications)

تحوير التركيب البروتيني ثلاثي الأبعاد (Three dimensional structure modifications)

Error and Damage Correction

فروقات بدء الترجمة بين بدائية وحقيقية النواة