Organization of Interrupted Genes Can Be Conserved

KEY CONCEPTS
-Introns can be detected when genes are compared with their RNA transcription products by sequencing.

-The positions of introns are usually conserved when homologous genes are compared between different organisms. The lengths of the corresponding introns can vary greatly.
-Introns usually do not encode proteins.
When a gene is uninterrupted, the map of its DNA corresponds with the map of its mRNA. When a gene possesses an intron, the map at each end of the gene corresponds to the map at each end of the message sequence. Within the gene, however, the maps diverge because additional regions that are found in the gene are not represented in the mature mRNA. Each such region corresponds to an intron. The example in FIGURE 1 compares the restriction maps of a β-globin gene and its mRNA. There are two introns, each of which contains a series of restriction sites that are absent from the complementary DNA (cDNA). The pattern of restriction sites in the exons is the same in both the cDNA and the gene. The finer comparison of the base sequences of a gene and its mRNA permits precise identification of introns. An intron usually has no open reading frame. An intact reading frame is created in an mRNA sequence by the removal of the introns from the primary transcript.

FIGURE 1 Comparison of the restriction maps of cDNA and genomic DNA for mouse βb-globin shows that the gene has two introns that are not present in the cDNA. The exons can be aligned exactly between cDNA and the gene.
The structures of eukaryotic genes show extensive variation. Some genes are uninterrupted and their sequences are colinear with those of the corresponding mRNAs. Most multicellular eukaryotic genes are interrupted, but the introns vary enormously in both number and size.
Genes encoding polypeptides, rRNA, or tRNA can all have introns.
Introns also are found in mitochondrial genes of plants, fungi, protists, and one metazoan (a sea anemone), as well as in chloroplast genes. Genes with introns have been found in every class of eukaryotes, Archaea, bacteria, and bacteriophages, although they are extremely rare in prokaryotic genomes.
Some interrupted genes have only one or a few introns. The globin genes provide a much-studied example . The two general classes of globin gene, α and β, share a common organization: They originated from an ancient gene duplication event and are described as paralogous genes or paralogs. The consistent structure of mammalian globin genes is evident from the “generic” globin gene presented in FIGURE 2.

FIGURE 2 .All functional globin genes have an interrupted structure with three exons. The lengths indicated in the figure apply to the mammalian βb-globin genes.
Introns are found at homologous positions (relative to the coding sequence) in all known active globin genes, including those of mammals, birds, and frogs. Although intron lengths vary, the first intron is always fairly short and the second is usually longer. Most of the variation in the lengths of different globin genes results from length variation in the second intron. For example, the second intron in the mouse α-globin gene is only 150 base pairs (bp) of the total
850 bp of the gene, whereas the homologous intron in the mouse major β-globin gene is 585 bp of the total 1,382 bp. The difference in length of the genes is much greater than that of their mRNAs (α-globin mRNA = 585 bases; β-globin mRNA = 620 bases). The example of the gene for the enzyme dihydrofolate reductase (DHFR), a somewhat larger gene, is shown in FIGURE 3. The mammalian DHFR gene is organized into six exons that correspond to a 2,000-base mRNA. The gene itself is long because the introns are very long. In three mammal species the exons are essentially the same and the relative positions of the introns are unaltered, but the lengths of individual introns vary extensively, resulting in a variation in the length of the gene from 25 to 31 kilobases (kb).

FIGURE 3. Mammalian genes for DHFR have the same relative organization of rather short exons and very long introns, but vary extensively in the lengths of introns.
The globin and DHFR genes are examples of a general phenomenon: genes that share a common ancestry have similar organizations with conservation of the positions (of at least some) of the introns.

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مواضيع ذات صلة

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The Serendipitous Discovery of trp Enzyme Hypersynthesis

Rapid Induction Capabilities of the trp Operon

The Aromatic Amino Acid Synthetic Pathway and its Regulation

How AraC Protein Loops and Unloops

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Binding Sites of the ara Regulatory Proteins

Endoplasmic Reticulum-Associated Degradation and the Unfolded Protein Response

Protein Folding and Modifications in the Endoplasmic Reticulum

Protein Modifications

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Repression by AraC