Without going into all the technical considerations, let’s see how the allele conferring a particular dominant disease trait (e.g., familial hypercholesterolemia) might be mapped. The first step is to obtain DNA samples from all the members of a family containing individuals that exhibit the disease. The DNA from each affected and unaffected individual is then analyzed to determine that individual’s genotype for a large number of known DNA polymorphisms (either STR or SNP markers can be used). The segregation pattern of each DNA polymorphism within the family is then compared with the segregation pattern of the disease under study. Poly morphisms that are not linked to the disease allele will not show any significant tendency to co-segregate along with the disease, whereas polymorphisms that are closely linked to it will almost always co-segregate with the disease, because recombination events that would separate the disease allele and the polymorphism will be rare. Computer analysis of the segregation data is used to calculate the likelihood of linkage between each DNA polymorphism and the disease-causing allele. Under ideal circumstances, the segregation patterns from at least 10 individuals in a family are needed to establish statistically significant evidence for linkage.

In practice, segregation data are usually collected from different families exhibiting the same disease and pooled. The more families that can be examined, the greater the statistical significance of any evidence for linkage that can be obtained, and the greater the precision with which the distance between a linked DNA polymorphism and a disease allele can be measured. Most family studies have a maxi mum of about 100 individuals in whom linkage between a disease gene and a panel of DNA polymorphisms can be tested. This number of individuals sets the practical upper limit on the resolution of such a mapping study to about 1 centimorgan, or a physical distance of about 7.5 × 10⁵ bp.

A phenomenon called linkage disequilibrium is the basis for an alternative mapping strategy, which can often afford a higher degree of resolution. This approach can be applied to a genetic disease commonly found in a particular population that results from a single mutation that occurred many generations in the past. The DNA polymorphisms carried by the ancestral chromosome in which the mutation occurred are collectively known as the haplotype of that chromosome. As the disease allele is passed from one generation to the next, only the polymorphisms that are closest to the disease gene will not be separated from it by recombination. After many generations, the region that contains the disease gene will be evident because it will be the only region of the chromosome that will carry the haplotype of the ancestral chromosome conserved through many generations (Figure 1). By assessing the distribution of specific markers in all affected individuals in a population, geneticists can identify DNA markers tightly associated with the disease, thus localizing the disease associated gene to a relatively small region. Under ideal circumstances, linkage-disequilibrium studies can improve the resolution of mapping studies to less than 0.1 centimorgans. The resolving power of this method comes from its ability to determine whether a polymorphism and the dis ease allele were ever separated by a meiotic recombination event at any time since the disease allele first appeared on the ancestral chromosome—in some cases, this can amount to finding markers that are so closely linked to the disease gene that even after hundreds of meioses, they have never been separated from it by recombination.

Fig1. Linkage-disequilibrium studies of human populations can be used to map genes at high resolution. A new disease mutation arises in the context of an ancestral chromosome among a set of polymorphisms known as the haplotype of that chromosome (indicated by red shading; the blue segments of chromosomes represent general haplotypes derived from the general population and not from the ancestral haplotype in which the mutation originally arose). After many generations, chromosomes that carry the disease mutation will also carry segments of the ancestral haplotype that have not been separated from the disease mutation by recombination. The regions closest to the disease mutation are the most likely to be the ancestral haplotype. This phenomenon is known as linkage disequilibrium. The position of the disease mutation can be located by scanning chromosomes that contain it for highly conserved polymorphisms corresponding to the ancestral haplotype.

اخر الاخبار

اخبار العتبة العباسية المقدسة

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بالتعاون مع العتبة العباسية المقدسة.. رابطة الأمين الثقافية تحتفي بذكرى ولادة الإمام المهدي (عجّل الله فرجه) في البصرة

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المزيد

الآخبار الصحية

عادات يومية تدمر قلبك بصمت.. خبراء يطلقون التحذير

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أطعمة تزيد فرص وفاة مرضى السرطان 60 بالمئة.. ما هي؟

اختبار ثوري جديد يكشف مبكرا عن سرطان البنكرياس

المزيد

الآخبار التكنلوجية

الذكور أم الإناث؟ دراسة "تنسف الشائع" بشأن التوحد

خبير يكشف عن اقتراب العلماء من فك شفرات "لغات الحيوانات" !

دراسة تدفعك لشراء ساعة ذكية "فورا".. هذا ما تقوله عن قلبك

علماء حاصلون على "نوبل": الذكاء الاصطناعي لن يستبدل الإنسان

المزيد

مواضيع ذات صلة

RNA Interference Causes Gene Inactivation by Destroying the Corresponding mRNA

DNA Polymorphisms Are Used as Markers for Linkage Mapping of Human Mutations

The Two- Hit Theory of Tumor Suppressor Gene Inactivation in Cancer

DNA Microarrays Can Be Used to Evaluate the Expression of Many Genes at One Time

Hybridization Techniques Permit Detection of Specific DNA Fragments and mRNAs

Cloned DNA Molecules Can Be Sequenced Rapidly by Methods Based on PCR

Cellular and Molecular Mechanisms in Development: Gene Regulation by Transcription Factors

Transcriptional Control of Megakaryocytopoiesis: GATA Family Transcription Factors

The Polymerase Chain Reaction Amplifies a Specific DNA Sequence from a Complex Mixture

Role of Developmentally Important Neutrophil-Specific Genes in Disease

Genes Can Be Identified by Their Map Position on the Chromosome

Homologous Recombination Can Repair DNA Damage and Generate Genetic Diversity